Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTOR/p70S6K/4E-BP1 Pathway

Figure 7

LY294002 inhibited cell-cycle progression induced by 0.25 mM LA in high glucose-cultured MCs. STO-609 prevented the inhibition of high glucose-stimulated mesangial cell-cycle progression caused by 1.0 mM LA. MCs were pretreated with high glucose (30 mM) for 1 h and then incubated with LY294004 (0, 2.5, 5, 10 μmol/L) plus 0.25 mM LA or STO-609 (0, 2.5, 5, 10 μg/mL) plus 1.0 mM LA, respectively, for an additional 24 h. Cells were analyzed by flow cytometry after PI staining. Data in the bar graphs represent the mean ± SD (). was considered statistically significant. Note: ^* versus NG; ^# versus HG. Abbreviations: NG, normal glucose (5 mM); Man (5 mM glucose + 25 mM mannitol); HG, high glucose (30 mM).

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