Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTOR/p70S6K/4E-BP1 Pathway

Figure 8

LY294002 prevented the secretions of FN and collagen-I induced by 0.25 mM LA in high glucose-cultured MCs. STO-609 reversed the inhibition of FN and collagen-I secretions induced by 1.0 mM LA in high glucose-treated MCs. (a) Representative of the effects of LY294002 on 0.25 mM LA induced FN and collagen secretion in supernatants. (b) Representative of the effects of STO-609 on 1.0 mM LA induced FN and collagen secretion in supernatants. Values are given as mean ± SD from 3 independent experiments in quadruplicate and is considered statistically significant. Note: ^* versus NG; ^# versus HG. Abbreviations: NG, normal glucose (5 mM); Man (5 mM glucose + 25 mM mannitol); HG, high glucose (30 mM).

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