Research Article
Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTOR/p70S6K/4E-BP1 Pathway
Table 1
Antibodies, their concentrations and manufacturers in the present studies.
| | Antibodies | Source | Dilutions | Manufacturer | Catalog number |
| |
P-AMPKα (Thr172) | Rabbit | 1 : 500 | Cell signaling | 4188S | | AMPKα | Rabbit | 1 : 1000 | Cell signaling | 4811S | | P-AKT (Ser473) | Rabbit | 1 : 200 | Cell signaling | 9271 | | AKT | Rabbit | 1 : 500 | Cell signaling | 9272 | | p-mTOR (Ser2448) | Rabbit | 1 : 500 | Cell signaling | 5536S | | mTOR | Rabbit | 1 : 1000 | Cell signaling | 2983S | | P-p70S6K (Thr389) | Rabbit | 1 : 500 | Cell signaling | 9234S | | p70S6K | Rabbit | 1 : 1000 | Cell signaling | 2708S | | P-4EBP1 (Thr70) | Rabbit | 1 : 500 | Cell signaling | 9455S | | 4E-BP1 | Rabbit | 1 : 1000 | Cell signaling | 9644S | | -Actin | Rabbit | 1 : 1000 | Santa Cruz | SC-130657 |
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