Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation

Figure 3

Western blotting of P50arom and ERα in the oviduct tissues of Rana dybowskii during the breeding period (B) and prehibernation (P). The oviductal content of estradiol-17 during the breeding period (B) and prehibernation (P). The positive bands of P450arom and ERα were observed in the position of about 55 kDa (a) and 66 kDa (b), respectively. β-actin blots were used as controls to correct for loading in each lane. The preabsorbed primary antibody was used instead of primary antibody for the negative control (lane NC). The estradiol-17 concentration was detected in both periods (c). NC: negative control. The expression levels were determined by densitometric analysis. Bars represent mean ± SD for three independent experiments. Mean values within the columns marked with asterisk were used instead of letters to indicate significant difference ().

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(b)

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