Simultaneous Quantification of 25-Hydroxyvitamin D₃ and 24,25-Dihydroxyvitamin D₃ in Rats Shows Strong Correlations between Serum and Brain Tissue Levels

Table 2

Calibration statistics.

Analytes	Regression equation		Linear range	LOQ
			(ng/mL)	(ng/mL)
Serum
25(OH)D3		0.9981	0.10–1000.00	0.10
24,25(OH)2D3		0.9992	0.25–333.30	0.25
Brain
25(OH)D3		0.9947	0.39–1250.00	0.10
24,25(OH)2D3		0.9979	0.47–1000.00	0.25