Simultaneous Quantification of 25-Hydroxyvitamin D₃ and 24,25-Dihydroxyvitamin D₃ in Rats Shows Strong Correlations between Serum and Brain Tissue Levels

Table 3

Intraday and interday precision and recovery of rat brain homogenates and serum.

Analytes	ADD	Intraday		Interday
	(ng/mL)	Accuracy %	CV %	Accuracy %	CV %
25(OH)D3
Serum	7.5	98.1	3.5	97.4	1.5
	60.0	103.3	5.0	102.5	3.3
	120.0	93.7	4.5	92.8	1.2
Brain	0.5	99.3	12.9	102.5	2.7
	7.5	102.0	9.2	101.1	1.4
	11.3	99.3	2.5	101.8	1.3
24,25(OH)2D3
Serum	7.5	98.2	4.3	97.5	3.3
	10.5	99.1	5.8	100.2	1.3
	21.0	98.0	7.1	98.0	1.9
Brain	1.1	100.9	6.5	103.9	1.7
	1.5	96.3	6.8	98.9	1.4
	3.0	96.3	4.8	98.7	1.5