Simultaneous Quantification of 25-Hydroxyvitamin D3 and 24,25-Dihydroxyvitamin D3 in Rats Shows Strong Correlations between Serum and Brain Tissue Levels
Table 4
The levels of targeted analytes and ratio of 25(OH)D3/24,25(OH)2D3 in brain homogenates and serum.
Analytes
Brain
Serum
Brain/serum
(ng/g)
(ng/mL)
25(OH)D3
LVD
1.01 ± 0.25
4.14 ± 0.72
0.26 ± 0.11
NVD
4.10 ± 1.16
14.61 ± 4.40
0.28 ± 0.07
HVD
13.34 ± 7.26
53.52 ± 8.89
0.25 ± 0.13
24,25(OH)2D3
LVD
0.46 ± 0.24
1.13 ± 0.50
0.44 ± 0.17
NVD
2.73 ± 1.68
4.37 ± 0.96
0.60 ± 0.28
HVD
6.84 ± 2.84
13.52 ± 5.74
0.52 ± 0.08
25(OH)D3/24,25(OH)2D3
LVD
2.64 ± 1.15
4.67 ± 2.97
—
NVD
1.80 ± 0.74
3.37 ± 0.61
—
HVD
1.99 ± 0.88
4.51 ± 1.77
—
Values are expressed as mean ± SD; for each group. LVD: low vitamin D3 group; NVD: normal vitamin D3 group; HVD: high vitamin D3 group. compared to the NVD group.
