Research Article
Effect of Orexin-A on Cortisol Secretion in H295R Cells via p70S6K/4EBP1 Signaling Pathway
Figure 2
Orexin-A stimulates the 4EBP1 kinase activity in H295R cells. Cells were exposed to orexin-A at concentration of 10−6 M for 0.25–24 h (a). Another treatment group consisted of 10−9 M–10−6 M orexin-A for 1 h (b). The expressions of 4EBP1 protein were measured via western blot analysis. Data are presented as mean ± SEM of three independent experiments. Asterisk indicates significant differences as compared to control ().
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