Research Article
Effect of Orexin-A on Cortisol Secretion in H295R Cells via p70S6K/4EBP1 Signaling Pathway
Figure 3
Orexin-A signals through the p70S6K/4EBP1 pathways. Cells were stimulated with orexin-A at concentration of 10−6 M, in the presence of PF-04691502 (10−6 M). Autophosphorylation of p-p70S6K/p-4EBP1 was evaluated along with the total protein activation. Protein activation was measured by western blot analysis. Data are presented as mean ± SEM of four independent experiments. Asterisks indicate significant differences compared to control samples ().
| (a) |
| (b) |