Methylation of Promoter Regions of Genes of the Human Intrauterine Renin Angiotensin System and Their Expression

Figure 2

The proportions of hypermethylated alleles of ACE, AGTR1, ATP6AP2, CTSD, and KLK1 and the mRNA abundances of these genes isolated from decidua collected from normal term pregnancies either by elective caesarean section prior to the onset of labour (white bars, ) or from women who had spontaneous labour and a vaginal delivery (grey bars, ). Significant differences were determined using Mann-Whitney tests, with significant differences denoted by * (). Data are presented as Tukey box plots. DNA methylation profiles could not be detected in the absence of labour for 1/8 ACE, 1/8 AGTR1, 2/8 ATP6AP2, and 3/8 KLK1 samples and from after labour for 3/8 KLK1 samples.