Ghrelin Increases Beta-Catenin Level through Protein Kinase A Activation and Regulates OPG Expression in Rat Primary Osteoblasts

Figure 2

Representative Western blot (a) and quantification (b) of timecourse experiments performed on rat osteoblast-like cells treated with ghrelin (AG, 10⁻¹⁰M) for 15, 30, 60, and 90 min. Phosphorylated-GSK-3β (P-GSK-3β) levels were measured in whole cell lysates and normalized to total GSK-3β levels (Tot-GSK3β). Results are shown as ratio of P-GSK3β measured in treated versus untreated cells. Data are the mean ± SEM of 5 experiments , versus untreated cells.

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