Effects of Ghrelin on Triglyceride Accumulation and Glucose Uptake in Primary Cultured Rat Myoblasts under Palmitic Acid-Induced High Fat Conditions

Table 1

Primers and PCR conditions used for RT-PCR.

Gene	Primers (5′-3′)	Gene location	Product length (bp)	Annealing temperature	Cycles
GLUT4	Forward: GGG CTG TGA GTG AGT GCT TTC; Reverse: CAG CGA GGC AAG GCT AGA	chr10	151	50°C	35
AMPK	Forward: TCA GGC ACC CTC ATA TAA TC; Reverse: TGA CAA TAG TCC ACA CCA GA	chr2	181	60°C	35
UCP3	Forward: ACG GAT GTG GTG AAG GTC CG; Reverse: TAC AAA CAT CAT CAC GTT CC	chr1	465	55°C	35
GAPDH	Forward: ATG GTG AAG GTC GGT GTG AAC; Reverse: GCT GAC AAT CTT GAG GGA GT	Multiple	437	52°C	35

GLUT4: glucose transporter 4; AMPK: AMP-activated protein kinase; UCP3: uncoupling protein 3; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.