[Retracted] Glycolipid Metabolism Disorder in the Liver of Obese Mice Is Improved by TUDCA via the Restoration of Defective Hepatic Autophagy

Figure 3

Effects of TUDCA on hepatic glucose and lipid metabolism in vivo. All analyses were performed in mice fed a normal diet (ND) or a high-fat diet (HFD) for 8 weeks and then injected daily with 500 mg/kg of TUDCA or with vehicle (Veh) for 8 weeks. (a) Relative expression of G6pase in liver (real-time PCR). (b) Relative expression of Pck1 in liver (real-time PCR). (c) Protein expression of G6pase and Pck1 in liver. (d) The relative protein quantity of G6pase and Pck1 in liver. (e) Fatty acid oxidation (pmol/g). (f) Relative expression of Pparα in liver (real-time PCR). (g) Relative expression of Cpt1α in liver (real-time PCR). (h) Relative expression of Fas in liver (real-time PCR). (i) Relative expression of Scd1 in liver (real-time PCR). The relative quantity of proteins was analyzed using Quantity One software. A representative blot is shown and the data was expressed as mean ± SEM in each bar graph. (HFD/TUDCA versus HFD/Veh). (HFD/Veh versus ND/Veh).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)