Research Article
Functional and Structural Consequences of Nine CYP21A2 Mutations Ranging from Very Mild to Severe Effects
Table 2
Apparent kinetic constants using 17-OHP as substrate.
| | | (pmol/mg protein per min) | (µM) | / |
| | Wild type | 519 (93) | 12.5 (1.3) | 42 (9) | | p.Leu12Met | 618 (207) | 17.7 (5.2) | 35 (4) | | p.Ala15Thr | 671 (145) | 16.9 (6.5) | 42 (8) | | p.Arg16Cys | 181 (31) | 5.0 (1.1) | 37 (3) | | p.Ser101Asn | 326 (67) | 9.7 (2.0) | 34 (4) | | p.Ser202Gly | 244 (47) | 8.0 (1.5) | 31 (5) | | p.Pro267Leu | 320 (93) | 8.4 (1.9) | 38 (7) | | p.Thr450Met | 43 (5) | 1.2 (0.1) | 35 (5) |
|
|
Values are present as the mean (1SD) of at least four experiments.
|
