Sex Hormone Binding Globulin Modifies Testosterone Action and Metabolism in Prostate Cancer Cells

Figure 3

Effects of testosterone and SHBG on LNCaP cell growth and morphology: (a) growth measured using the IncuCyte ZOOM Live Imaging System. LNCaP cells were treated with ((a)(A)) 0, 1, 25, and 100 nM testosterone (Te) in serum-free medium, ((a)(B)) 25 nM Sex Hormone Binding Globulin (SHBG) plus 0, 1, 25, and 100 nM testosterone in serum-free medium, and ((a)(C)) 125 nM SHBG plus 0, 1, 25, and 100 nM testosterone in serum-free medium. Measurements gathered over 86 hours without changing medium. Values are expressed as mean percentage phase object confluence using 9 nonoverlapping images per well. (b) Morphology assessed by IncuCyte Imaging. Cells were imaged after 86 hours in culture using IncuCyte ZOOM Live Imaging System. ((b)(A)) LNCaP cells cultured in serum-free medium were used as the normal control. ((b)(B)) Cells treated with 25 nM testosterone only and ((b)(C)) cells treated with 25 nM testosterone plus 125 nM SHBG. Bar = 50 μm.