Androgen Receptor Localizes to Plasma Membrane by Binding to Caveolin-1 in Mouse Sertoli Cells

Figure 3

Knockdown of caveolin-1 decreased AR membrane localization and translocation. Results of representative Western blot analysis (a) and real-time PCR (b) show caveolin-1 knocked down by shRNA transfection. (c) Representative Western blot analysis indicating that AR trafficking under 10 nM testosterone treatment in membrane and nuclear fractions from cells transfected with negative control shRNA or caveolin-1 shRNA. (d) The graph shows the mean and SEM of AR expression in the membrane fraction from 4 experiments. Values are expressed as fold-change of the negative control basal. .

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