Simulated Cholinergic Reinnervation of β (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing β Cell and Cholinergic Cell

Figure 5

Glucose-stimulated insulin secretion assay. (a) Secretory responses of INS-1 cells with or without carbachol (10 μM) or atropine sulfate (10 μM). and ns compared to INS-1 group, . (b) Secretory responses of INS-1 cells and 2D coculture cells. and ns compared to INS-1 group, . (c) Secretory responses of INS-1 PIs and INS-1 PIs + NG108-15 clusters mixture and heterotypic PIs. and ns compared to INS-1 PI group under 20 mM glucose stimulation, . (d) Stimulation index of all groups. compared to 2D INS-1 group. compared to INS-1 PI groups. ns compared to 2D INS-1 group. (a, b, and c) The data are expressed as percentages of total insulin content. Results are means ± SD of at least four independent experiments.

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