Beta-Arrestin 1 Mediates Liver Thyrotropin Regulation of Cholesterol Conversion Metabolism via the Akt-Dependent Pathway

Figure 5

Immunoblotting analysis of cholesterol metabolism-related proteins in 24-hour TSH treatment. Cells were starved overnight and treated with 4 μM TSH. Total protein was extracted at 0, 6, 12, and 24 hours after TSH was added. (a) HepG2-Cas9 cells, (b) ARRB1-knockdown HepG2-Cas9 cells, (c) ARRB2-knockdown HepG2 cells, and (d, e) grayscale analysis of relative expression level of HMGCR and CYP7A1 in TSH treatment of 0, 6, 12, and 24 hours. indicated a statistical significance between genotypes. Error bars are calculated as a standard error (SEM). (f) Differences of HMGCR and CYP7A1 expression levels between ARRB1/2-knockdown and wild-type HepG2 cells with or without TSH treatment for 24 hours. (g, h) Grayscale analysis of HMGCR and CYP7A1 expression levels between ARRB1/2-knockdown and wild-type HepG2 cells treated with or without 24 h TSH treatment. indicated a statistical significance between time points within genotypes. indicated a statistical significance between time points within genotypes.

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