A Possible Mechanism of Metformin in Improving Insulin Resistance in Diabetic Rat Models

Figure 1

Cell viability and protein expression of FXR, MAFG, and CYP8B1 in each group by metformin treatment in HepG2 cells. (a) The effect of different doses of metformin (Met) on cell viability. Cells were treated with metformin for 96 hours, and the cell viability was measured by CCK8 assay. Results are mean ± SEM; vs. control group. Protein contents were detected by western blotting and were quantified with Image J β-actin was used as a reference to verify equal loading of protein sample. The HepG2 cells were incubated with different concentrations of metformin (0, 0.5, 1, 1.5, and 2 mM) for 24 h quantification of FXR (b), MAFG (c), and CYP8B1 (d). The HepG2 cells were incubated with 1 mM metformin for various durations (0, 12, 24, or 48 h), quantification of FXR (e), MAFG (f), and CYP8B1 (g). Results are mean ± SEM; vs. control group.

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