miR-98-5p Alleviated Epithelial-to-Mesenchymal Transition and Renal Fibrosis via Targeting Hmga2 in Diabetic Nephropathy

Figure 5

Overexpression of miR-98-5p alleviated high glucose-induced cell proliferation, EMT, and renal fibrosis in cultured mice mesangial cells. (a) Real-time qPCR analysis showing miR-98-5p expression in mice mesangial cells treated with high glucose (HG, 25 mM) as compared with the cells treated with normal glucose (NG, 5.6 mM). (b) Real-time qPCR analysis showing miR-98-5p expression in mice mesangial cells infected with LV-miR-98. (c) Result of CCK-8 assay in mouse mesangial cells. (d and e) Result of EDU assay in mouse mesangial cells. Western blot images of E-cadherin and N-cadherin (f). Quantification of the western blot images of E-cadherin and N-cadherin (g and h). The western blot images of TGF-β1 and COL4A1 (i). Quantification of the western blot images of TGF-β1 (j) and COL4A1 (k). for each group. Data are representative of three experiments. Error bars stand for the mean ± SD of at least triplicate experiments. .

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