miR-98-5p Alleviated Epithelial-to-Mesenchymal Transition and Renal Fibrosis via Targeting Hmga2 in Diabetic Nephropathy

Figure 6

HMGA2 was a direct target of miR-98-5p. (a) The luciferase reporter constructs containing the wild type (WT-HMGA2) or mutant HMGA2 (MUT-HMGA2) sequence. (b) WT-HMGA2 or MUT-HMGA2 was cotransfected into HEK-293T cells with miR-98-5p mimics or their corresponding negative controls. (c) mRNA expression of HMGA2 in db/db mice. (d) Protein expression of HMGA2 in db/db mice. (e) mRNA expression of HMGA2 in mouse mesangial cells. (f) Protein expression of HMGA2 in mouse mesangial cells. (g) mRNA expression of HMGA2 in mouse mesangial cells transfected with LV-miR-98-5p. (h) Protein expression of HMGA2 in mouse mesangial cells transfected with LV-miR-98-5p. Data are representative of three experiments. Error bars stand for the mean ± SD of at least triplicate experiments. .

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