3-Deoxyglucosone Induces Glucagon-Like Peptide-1 Secretion from STC-1 Cells via Upregulating Sweet Taste Receptor Expression under Basal Conditions

Figure 1

Acute effects of 3DG on basal GLP-1 secretion in vivo and in STC-1 cells. Saline (control) or 3DG at 50 mg/kg was administered to the fasted rats by gastric gavage. Blood samples were collected from the aorta abdominals before (0 min) and after (15, 30, 60, and 120 min) 3DG administration. (a) Plasma GLP-1 concentration was measured by ELISA and (b) the area under curves for plasma GLP-1 was evaluated. , compared with the control group at the same point (a), compared with the control group (b) (n = 3). (c) Saline (control) or 3DG at 50 mg/kg was administered to the fasted rats by gastric gavage. After one hour, the duodenum tissue was obtained for the measurement of 3DG content. (n = 3). (d) STC-1 cells were incubated for 1 h with 5.6 mM glucose in the presence and absence of 3DG. The GLP-1 concentration in the supernatant was measured by ELISA. The data are expressed as a percent of the untreated group. (n = 6).

(a)

(b)

(c)

(d)