3-Deoxyglucosone Induces Glucagon-Like Peptide-1 Secretion from STC-1 Cells via Upregulating Sweet Taste Receptor Expression under Basal Conditions

Figure 3

Possible involvement of STRs, SGLT1, or GLUT2 in 3DG-induced acute GLP-1 secretion in STC-1 cells under glucose-free conditions. (a) STC-1 cells were incubated for 1 h with 0 or 300 ng/ml 3DG in the presence and absence of lactisole (5 mM), phloretin (0.1 mM), and phloridzin (0.5 mM) under glucose-free conditions. The GLP-1 concentration in the supernatant was measured by ELISA. The data are expressed as a percent of the untreated group. , (n = 6). (b) STC-1 cells were incubated for 1 h with 0 mM glucose in the presence and absence of 300 ng/ml 3DG. The protein expression of STR subunits (TAS1R2 and TAS1R3) and TRPM5 was measured. (c) The protein levels of TAS1R2, TAS1R3, and TRPM5 were analyzed by ImageJ software. , (n = 3).

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