Hyperoside Alleviates High Glucose-Induced Proliferation of Mesangial Cells through the Inhibition of the ERK/CREB/miRNA-34a Signaling Pathway

Figure 1

Hyperoside inhibits HG-induced SV40-MES13 cells proliferation by downregulating miR-34a. (a) Time-dependent effect and dose-dependent (50, 100, 200 μM) effect of hyperoside on viable cell count of SV40-MES13 cells, as determined by CCK-8. (b) Cell apoptosis were analyzed by flow cytometry with the PE-Annexin V/7AAD staining. Representative data from triplicate experiments are shown. (c) SV40-MES13 cells were treated with LG or HG with different concentration of hyperoside for 48 h. The miR-34a expression was determined by qRT-PCR. (d) SV40-MES13 cells were transfected with agomir-34a or agomir-NC and then cultured with HG for 48 h with or without 200 μM hyperoside. Cell proliferation was determined by CCK-8. (e) Cell proliferation rates were detected by EdU incorporation and the quantitative analysis (; , ). LG: low glucose (5.5 mM); HG : high glucose (30 mM); and HYP: hyperoside.

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