TET1 is a Tumor Suppressor That Inhibits Papillary Thyroid Carcinoma Cell Migration and Invasion

Figure 2

5hmC expression in PTC tissues and cell lines. (a, b) 5hmC level significantly decreased in PTC tissues compared with thyroid benign nodule tissues as detected by immunohistochemical analysis. Brownish-yellow is the color of 5hmC, and blue is the color of hematoxylin-stained nuclei. The staining range and degree of 5hmC were significantly weaker in PTC tissues than in thyroid benign nodule tissues. (c) Dot blot assay showed the expression level of 5hmC in PTC cell lines when the DNA sample size was 200 ng and 400 ng (left). Methylene blue staining of the identical membrane was used as the sample control (right). (d) ImageJ software was used to analyze exposure grayscale; it showed that the expression level of 5hmC in PTC cell lines decreased compared with the normal Nthy-ori 3-1 cells. The data represented the mean ± SD from three independent experiments. , , and .

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