TET1 is a Tumor Suppressor That Inhibits Papillary Thyroid Carcinoma Cell Migration and Invasion

Figure 3

TET1 inhibited the invasion and migration in BCPAP cells. (a, b) TET1 level significantly declined after transfection with si-TET1 RNAs as detected using qRT-PCR and Western blot assays. (c) Dot blot assay showed the expression of 5hmC after 48 h of transfection of si-TET1 RNAs (left). Methylene blue staining of the identical membrane was used as the sample control (right). (d) ImageJ software was used to analyze exposure grayscale and showed that the downregulated TET1 expression level was consistent with the reduction in the 5hmC level. (e) EdU incorporation assay was conducted to examine the potential role of TET1 in cell proliferation after si-TET1 and si-TET2 transfection. Fluorescent images of proliferative cells (red) stained with EdU and nuclei (blue) counterstained with DAPI in BCPAP cells. Magnification, 400x. (f) Quantification of EdU incorporation assay in BCPAP cells. (g) Transwell migration and invasion assays of BCPAP cells after si-TET1 and si-TET2 transfection were performed without and with the Matrigel-coated chamber. Magnification, 200x. (h) Quantification of migrated cells. (i) Diagrams of invasive cells. , , and .

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