Islet Stellate Cells Regulate Insulin Secretion via Wnt5a in Min6 Cells

Figure 6

Intracellular mechanism of action of Wnt5a in the regulation of insulin secretion from Min6 cells. (a) GSIS was induced, and qRT-PCR analyses of Min6 cells alone, Min6 cells cultured with ISC supernatant, and Min6 cells cultured with exogenous Wnt5a were performed to measure β-catenin, Ror2, CamKII, FoxO1, PDX1, Glut2, and Cask mRNA level (2 mM glucose conditions). and vs. Min6 cells cultured alone; (b) GSIS was induced, and β-catenin, Ror2, pCamKII, pFoxO1, FoxO1, PDX1, Glut2, and Cask protein levels were quantified by western blotting of Min6 cells cultured alone, Min6 cells cultured with ISC supernatant, and Min6 cells cultured with exogenous Wnt5a (2 mM glucose conditions). and vs. Min6 cells cultured alone.

(a)

(b)