Older Age Is Associated with Decreased Levels of VDR, CYP27B1, and CYP24A1 and Increased Levels of PTH in Human Parathyroid Glands

Figure 2

Immunohistochemical staining of (a) VDR, (b) CYP27B1, (c) CYP24A1, and (d) PTH in human parathyroid glands. The specimens from seventy parathyroid glands were fixed in formalin solution and embedded in paraffin blocks for routine histological and immunohistochemical analysis using antibodies against VDR, CYP27B1, CYP24A1, or PTH. Immunohistochemical staining of VDR, CYP27B1, CYP24A1, or PTH is shown in brown, and the counterstaining is shown in blue in the representative section. VDR is located in the nucleus. CYP27B1 and CYP24A1 are located in the cytoplasm, and PTH is located in the cytoplasm and plasma membrane. PBS instead of the primary antibody was used as a negative control. Immunohistochemical staining of VDR in the epidermis, CYP24A1 in the kidney, and CYP27B1 in liver was used as positive controls, respectively (data not shown).

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