Effects of Dendrobium Polysaccharides on the Functions of Human Skin Fibroblasts and Expression of Matrix Metalloproteinase-2 under High-Glucose Conditions

Table 1

Specific primer sequences for RT-qPCR.

Gene		Sequence (5′-3′)	Product length (bp)
TIMP-2	Forward	CCTCTGGCATCTCTTGT	423
	Reverse	ACCCCACAGCCAGCACTAT
Bcl-2	Forward	TTCTGGGCAACA AGTATGA	436
	Reverse	GTCACTGTCCGCCAAATA
GAPDH	Forward	TCGGACGCCTGGTTAC	199
	Reverse	CGCT CCTGGA AGATGG

Primer design: the target gene sequence was searched in the NCBI. Primer 5 software was used to design the primer.