miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia

Figure 1

HepG2 cells were treated with different doses of TNF-α to detect the optimum dose. After detecting the safe doses of TNF-α in HepG2 cells, we incubated the cells with 4 different doses of TNF-α. Epo mRNA was inhibited by TNF-α in all doses but in 20 ng/ml dose inhibited Epo in the medium more significantly. Control: treated with normal medium. 1 ng, 5 ng, 10 ng, 15 ng, 20 ng, 25 ng, 30 ng, 40 ng, 50 ng, 75 ng, and 100 ng: treated with different doses of TNF-α. and . (a) MTT. (b) Epo mRNA qRT-PCR analysis. (c) ELISA analysis.

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