miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia

Figure 4

Results of the coculture study. When HepG2 cells were treated with 30 pmol mimic-miR663a, they synthesized enough Epo to let the UT-7/Epo cells survive, and when HepG2 cells were only treated with TNF-α, UT-7/Epo cells showed apoptosis in our coculture experiments. Both in normoxia and hypoxia showed similar results. Control: treated with normal medium; TNF-α: treated with 20 ng/ml TNF-α; and miR663: treated with 20 ng/ml TNF-α and 5 pmol mimic-miR663a. and . (a) Control hypoxia 40X. (b) miR663 hypoxia 40X. (c) TNF-alpha hypoxia 40X. (d) TUNEL analysis of UT-7 Epo cells cocultured with HEPG2 in normoxia. (e) Control hypoxia 40X. (f) miR663 hypoxia 40X. (g) TNF-alpha hypoxia 40X. (h) TUNEL analysis of UT-7 Epo cells cocultured with HEPG2 in hypoxia.

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