Overexpression of PAX8-AS1 Inhibits Malignant Phenotypes of Papillary Thyroid Carcinoma Cells via miR-96-5p/PKN2 Axis

<div>Overexpression of PAX8-AS1 impedes the cell proliferation and enhances apoptosis. <i>Note.</i> (a) The transfection efficiency of PAX8-AS1 in PTC K1 and IHH4 cells was detected by qRT-PCR; (b) MTT assay was conducted to separately detect the optical density values at 490 nm at 24, 48, and 72 h in K1 and IHH4 cells after overexpressing PAX8-AS1; (c) cell proliferation was examined in K1 and IHH4 cells after PAX8-AS1 overexpression by EdU assay; (d) flow cytometry was used to analyze the scatter patterns of cell cycle distribution of K1 and IHH4 cells after PAX8-AS1 overexpression; (e) the protein bands of apoptosis-related proteins in K1 and IHH4 cells were detected by western blot. <span class="nowrap"><svg height="11.6856pt" id="M3" style="vertical-align:-1.5831pt" version="1.1" viewbox="-0.0498162 -10.1025 209.803 11.6856" width="209.803pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path 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International Journal of Endocrinology

fig2

Figure 2

Figure 2: Overexpression of PAX8-AS1 Inhibits Malignant Phenotypes of Papillary Thyroid Carcinoma Cells via miR-96-5p/PKN2 Axis 