Overexpression of PAX8-AS1 Inhibits Malignant Phenotypes of Papillary Thyroid Carcinoma Cells via miR-96-5p/PKN2 Axis

<div>Interference with PKN2 reverses the effect of overexpression of PAX8-AS1 on cell proliferation and apoptosis. <i>Note.</i> (a) The expression of PKN2 in PTC K1 cells in vector, PAX8-AS1, and PAX8-AS1+sh-PKN2 groups was detected by qRT-PCR and western blotting; (b) the optical density values at 490 nm at 24, 48, and 72 h in PTC K1 cells in the above three groups were detected by MTT assay; (c) cell proliferation of PTC K1 cells in the above three groups was examined by EdU assay; (d) flow cytometry was used to analyze the scatter patterns of cell cycle distribution of K1 cells in the above three groups; (e) the protein bands of apoptosis-related proteins in K1 cells in the above three groups were detected by western blot. <span class="nowrap"><svg height="11.6791pt" id="M7" style="vertical-align:-1.5766pt" version="1.1" viewbox="-0.0498162 -10.1025 211.982 11.6791" width="211.982pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g 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International Journal of Endocrinology

fig6

Figure 6

Figure 6: Overexpression of PAX8-AS1 Inhibits Malignant Phenotypes of Papillary Thyroid Carcinoma Cells via miR-96-5p/PKN2 Axis 