MEK1/2 Inhibitor (GDC0623) Promotes Osteogenic Differentiation of Primary Osteoblasts Inhibited by IL-1β through the MEK-Erk1/2 and Jak/Stat3 Pathways

Figure 7

Effects of C188-9 on expressions of osteogenic marker genes, ALP staining, and ALP activities. Osteoblasts were cultured in an osteogenic induction medium containing IL-1β or IL-1β plus C188-9 for one week. (a) qPCR analysis of osteogenic gene expressions in osteoblasts was performed, relative to internal control GAPDH, and normalized to control cells. (b) ALP staining (upper) and details of staining (lower) among the three groups. (c) Data are expressed as mean ± SD for n = 3.  < 0.05 versus the control group,  < 0.05 versus the IL-1β group.

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