MEK1/2 Inhibitor (GDC0623) Promotes Osteogenic Differentiation of Primary Osteoblasts Inhibited by IL-1β through the MEK-Erk1/2 and Jak/Stat3 Pathways

Table 1

Primers used for real-time PCR.

Gene name	GenBank accession no.	mRNA sequences (5′-3′)
GAPDH-F		TTCAACGGCACAGTCAAGG
	NM_017008.4
GAPDH-R		CTCAGCACCAGCATCACC
OCN-F		CCCAATTGTGACGAGCTAGC
	M25490.1
OCN-R		CTGTGCCGTCCATACTTTCG
Runx2-F		AAGGAGCACAAACATGGCTG
	NM_001278483.1
Runx2-R		TCTTAGGGTCTCGGAGGGAA
Osterix-F		CAAHGGTTAGGTGGTGGGC
	AY177399.1
Osterix-R		TCTTGGGGTAGGACATGCTG
OPN-F		CGGTGAAAGTGGCTGAGTTT
	M14656.1
OPN-R		GGCTACAGCATCTGAGTGTTTG
OPG-F		TGAGGTTTCCAGAGGACCAC
	U94330.1
OPG-R		GGAAAGGTTTCCTGGGTTGT
COL1α1-F		CATGTTCAGCTTTGTGGACCT
	NM_053304.1
COL1α1-R		GCAGCTGACTTCAGGGATGT
ALP-F		GCACAACATCAAGGACATCG
	NM_013059.1
ALP-R		TCAGTTCTGTTCTTGGGGTACAT