FOXA3, a Negative Regulator of Nur77 Expression and Activity in Testicular Steroidogenesis

Figure 3

FOXA3 represses cAMP-induced CREB transactivation and expression in MA-10 cells. FOXA3 strongly inhibits cAMP-induced CREB transactivation of a CRE-dependent promoter in MA-10 cells. Cells were transfected with a CRE-Luc reporter, SV-40 β-gal, and indicated concentration of FOXA3 (X represents 100 ng of the expression construct) for 48 h that included overnight cAMP induction (200 μM). Luciferase activity was normalized to β-galactosidase activity (A). FOXA3 represses CREB expression in MA-10 cells. Foxa3 and Creb expression were quantified using qRT-PCR. The gene expression level was normalized to that of GAPDH. Cells were transfected with indicated concentrations of FOXA3 (X represents 200 ng of the expression construct) for 48 h that included cAMP induction (200 μM) for 4 h (B). Statistical significance was assessed using the t-test. ; .

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