FOXA3, a Negative Regulator of Nur77 Expression and Activity in Testicular Steroidogenesis

<div>FOXA3 regulates the expression of Nur77 and steroidogenic genes. Expression of Nur77 and steroidogenic genes in MA-10 cells transfected with pcDNA3-only or pcDNA3-FOXA3 (X represents 200 ng of the expression construct). After 44 h of transfection, cells were treated with 200 <i>μ</i>M cAMP for 4 h. Gene expression was quantified by qRT-PCR, and the gene expression level was normalized to that of GAPDH. 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602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,55.606,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,61.846,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,68.086,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> compared to cAMP-induced samples.</div>

International Journal of Endocrinology

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Figure 4

Figure 4: FOXA3, a Negative Regulator of Nur77 Expression and Activity in Testicular Steroidogenesis 