FOXA3, a Negative Regulator of Nur77 Expression and Activity in Testicular Steroidogenesis

<div>FOXA3 represses cAMP-induced expression of steroidogenic genes in primary Leydig cells. mRNA expression of steroidogenic genes in primary Leydig cells (A). Mouse primary Leydig cells were transduced with Ad-FOXA3 or Ad-GFP as control. After wash, the cells were further cultured for 48 h that included 4 h of cAMP (200 <i>μ</i>M) induction. The gene expression level was normalized to that of GAPDH. Testosterone production was significantly reduced by FOXA3 (B). Cells were transduced in a similar manner and maintained in 200 <i>μ</i>M of cAMP for 48 h Culture medium was harvested for RIA assay. 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International Journal of Endocrinology

fig6

Figure 6

Figure 6: FOXA3, a Negative Regulator of Nur77 Expression and Activity in Testicular Steroidogenesis 