Discovery of a Novel Variant of SEMA3A in a Chinese Patient with Isolated Hypogonadotropic Hypogonadism

Figure 2

Functional study of the novel SEMA3A variant. (a) Expression and secretion of WT SEMA3A protein and T457A mutant protein. (b) Above: GN11 cells incubated with the serum-free medium (mock, negative control) or conditioned medium containing WT and mutated SEMA3A protein for 20 minutes. The phosphorylated FAK (pFAK) and total FAK in GN11 cells detected by Western blot. Below: the bar chart shows the ratio of pFAK to total FAK. . (c) Above: transwell assay of GN11 cells incubated with the serum-free medium (pRK5, negative control) or the conditioned medium containing WT and mutated SEMA3A protein. Below: the statistic analysis of the transwell assay . Scale bars: 30 mm.

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