Identification of Three Significant Genes Associated with Immune Cells Infiltration in Dysfunctional Adipose Tissue-Induced Insulin-Resistance of Obese Patients via Comprehensive Bioinformatics Analysis

Figure 6

Hub genes’ upregulation in AT was accompanied with process of obtained obesity-induced IR. Validation of CSF1R, C1QC, and TYROBP gene expressions levels by using RTqPCR. (a) Body weight curves of HFD feeding (n = 7) and ND feeding controls (n = 7) mice. Glucose tolerance tests (GTT) were performed in HFD fed mice and ND fed mice after 12 weeks feeding period. (b) mRNA expression levels of the top 3 hub genes in adipose tissue of SAT or VAT from HFD feeding mice and ND feeding control mice after 12 weeks (n = 7). (c) mRNA expression levels of the top 3 hub genes in adipose tissue of SAT or VAT from IRO patients and IS controls (n = 5). Unpaired two-sided t test, value <0.05. value <0.01. value <0.001. n.s. = not significant.

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