Constitutive Androstane Receptor-Mediated Inhibition of Metformin on Phase II Metabolic Enzyme SULT2A1

Figure 3

The effects of metformin with or without CITCO on CAR protein expression. HepaRG cells were treated with metformin (0.1, 0.5, 1 mM) (a) and CITCO (1 μM) (b) either individually or in combination for 48 h. Cells treated only with DMSO were considered as the control group. Total cell proteins and the nuclear proteins were harvested, respectively. Protein levels were determined by western blotting and were quantified with Image J. Quantitation of the T-CAR protein bands was normalized to β-actin; quantitation of the N-CAR protein bands was normalized to lamin-B1. The data shown are the mean ± SEM (n = 3) ( and , comparison indicated by brackets. ns: not significant).

(a)

(b)