Constitutive Androstane Receptor-Mediated Inhibition of Metformin on Phase II Metabolic Enzyme SULT2A1

Figure 4

The effects of metformin on the levels of RXRα, HNF4α, and PGC-1α in HepaRG cells. HepaRG cells were treated with metformin (1 mM) and CITCO (1 μM), either individually or in combination for 48 h. Cells treated only with DMSO were considered as the control group. Whole-cell extracts were harvested, and the expressions of RXRα, HNF4α, PGC-1α, and the internal control β-actin were analyzed using western blotting. Quantitation of the three protein bands was normalized to β-actin, respectively. A representative blot was shown and quantified by Image J software. The data shown are the mean ± SEM (n = 3).