CREB3 Transactivates lncRNA ZFAS1 to Promote Papillary Thyroid Carcinoma Metastasis by Modulating miR-373-3p/MMP3 Regulatory Axis

Figure 4

ZFAS1 modulated miR-373-3p/MMP3 axis. (a) MMP3 was predicted as a direct target of miR-373-3p. (b) Target between MMP3 and miR-373-3p was confirmed by luciferase assay (). (c) The effect of miR-373-3p on MMP3 expression in TPC-1 cell was tested by q-PCR (). (d) MMP3 protein level was decreased by miR-373-3p mimics (). (e) miR-373-3p and MMP3 expression were tested by q-PCR after ZFAS1 knocking down in TPC-1 cells (, ). (f) MMP3 expression profile in primary tumor and normal tissues obtained from TCGA-THCA datasets (). (g) The effect of MMP3 knocking down on ZFAS1 expression was tested by q-PCR. (h) ZFAS1 and MMP3 enrichment with AGO2 antibody analyzed by RNA immunoprecipitation followed q-PCR test after ectopically expressed miR-373-3p ().

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)