Detection of BRAF V600E in Fine-Needle Aspiration Samples of Thyroid Nodules by Droplet Digital PCR

Table 1

Comparison of the results of Sanger sequencing and ddPCR for BRAF V600E in 310 nodules with FNAB cytology results.

Bethesda categories	Cytologic reporting	N	ddPCR(+)	SS(+)
All	All	310	94	40
Bethesda I	Nondiagnostic	3	0	0
Bethesda II	Benign	161	2	0
Bethesda III	Follicular adenoma	13	1	0
Bethesda V	Suspicious for PTC	15	2	0
Bethesda VI	PTC	117	89	40
Bethesda VI	Medullary carcinoma	1	0	0

PTC, papillary thyroid carcinoma; ddPCR, droplet digital PCR; SS, sanger sequencing.