Detection of BRAF V600E in Fine-Needle Aspiration Samples of Thyroid Nodules by Droplet Digital PCR

Table 2

Comparison of the results of Sanger sequencing and ddPCR for BRAF V600E in 191 nodules with pathology results.

FNAB		Surgery
Bethesda categories	Cytologic reporting	N	PTC	ddPCR(+)	SS(+)	Non-PTC	ddPCR(+)	SS(+)
All	All	191	119	80	31	72	0	0
Bethesda I	Nondiagnostic	2	0	0	0	2	0	0
Bethesda II	Benign	59	3	0	0	56	0	0
Bethesda III	Follicular adenoma	12	0	0	0	12	0	0
Bethesda V	Suspicious for PTC	11	10	2	0	1	0	0
Bethesda VI	PTC	106	106	78	31	0	0	0
Bethesda VI	Medullary carcinoma	1	0	0	0	1	0	0

PTC, papillary thyroid carcinoma; ddPCR, droplet digital PCR; SS, sanger sequencing; non-PTC, including benign nodules, follicular adenoma, and medullary carcinoma.