Embryo Density and Its Neutrality in Day-3 Embryo Development: A Retrospective Cohort Study
Table 3
Univariate analysis for cleaving speed, quality, and clinical results of day-3 embryos.
Variables
7–10 cell embryosa
MGEb
Implanted embryosc
EXP (OR) 95% CI
value
EXP (OR) 95% CI
value
EXP (OR) 95% CI
value
Embryo density (ul/embryo)
30
1.04 (0.91, 1.19)
0.55
0.98 (0.83, 1.15)
0.78
1.38 (0.79, 0.41)
0.25
15
1.09 (0.97, 1.22)
0.15
0.96 (0.83, 1.10)
0.53
1.31 (0.77, 2.28)
0.31
10
Reference
Reference
Reference
Maternal age, y
0.99 (0.98, 1.01)
0.26
0.99 (0.98, 1.01)
0.22
0.93 (0.90, 0.97)
<0.01
Paternal age, y
1.00 (0.99, 1.01)
0.501
0.99 (0.98, 1.00)
0.20
0.94 (0.91, 0.97)
<0.01
Maternal BMI, kg/m2
1.00 (0.99, 1.01)
0.617
0.99 (0.97, 1.00)
0.14
1.04 (0.99, 1.08)
0.098
Type of infertility
Primary
1.11 (0.99, 1.23)
0.06
1.17 (1.03, 1.34)
0.02
1.32 (0.95, 1.85)
0.099
Secondary
Reference
Reference
Reference
AMH
1.02 (1.00, 1.03)
0.04
1.01 (0.99, 1.03)
0.401
1.08 (1.01, 1.16)
0.04
Antral follicles
1.00 (0.99, 1.01)
0.49
1.00 (0.99, 1.01)
0.84
1.03 (0.96, 1.10)
0.43
COS protocol
Long and antagonist
1.01 (0.88, 1.17)
0.89
0.91 (0.76, 1.09)
0.33
1.35 (0.79, 2.29)
0.27
Other protocols
Reference
Reference
Reference
Length of stimulation
1.01 (0.99, 1.03)
0.45
1.01 (0.98, 1.03)
0.75
1.08 (1.00, 1.16)
0.047
No. of retrieved oocytes
1.00 (1.00, 1.03)
0.06
1.01 (1.00, 1.02)
0.09
1.01 (0.97, 1.05)
0.58
Type of insemination
IVF
0.97 (0.87, 1.07)
0.53
0.94 (0.83, 1.07)
0.36
1.17 (0.83, 1.64)
0.38
ICSI
Reference
Reference
Reference
Incubator
COOKA
1.07 (0.88, 1.30)
0.52
1.11 (0.86, 1.44)
0.42
1.03 (0.54, 1.96)
0.94
COOK8
1.15 (0.94, 1.41)
0.16
1.35 (1.04, 1.75)
0.03
0.98 (0.51, 1.88)
0.94
COOK7
1.12 (0.88, 1.41)
0.36
1.07 (0.80, 1.42)
0.67
1.35 (0.60, 3.03)
0.46
COOK6
0.96 (0.79, 1.16)
0.66
1.06 (0.84, 1.4)
0.62
1.46 (0.80, 2.67)
0.22
COOK5
1.02 (0.85, 1.24)
0.81
1.08 (0.85, 1.4)
0.53
1.05 (0.56, 1.99)
0.88
COOK3
1.07 (0.88, 1.30)
0.50
1.20 (0.93, 1.5)
0.16
0.73 (0.38, 1.40)
0.34
COOK2
0.98 (0.81, 1.2)
0.87
1.14 (0.90, 1.5)
0.28
0.83 (0.44, 1.57)
0.57
COOK1
Reference
Reference
Reference
Culture medium
Vitrolife
1.10 (0.98, 1.22)
0.10
1.19 (1.04, 1.37)
0.02
1.36 (0.96, 1.93)
0.09
Cook
Reference
Reference
Reference
BMI: body mass index; AMH: anti-Müllerian hormone; COS: controlled ovarian stimulation; IVF: in vitro fertilization and embryo transfer; ICSI: intracytoplasmic sperm injection; MGE: morphological good embryo. aWhether the embryo cleaved to 7–10 cells on day 3. bWhether the embryo was a MGE on day 3. cWhether the transferred embryo was successfully implanted. Analysis done by generalized estimate equation (GEE) model, subject ID = patient unique medical record number (independence).
