PNPLA3 148M/M Is More Susceptible to Palmitic Acid-Induced Endoplasmic Reticulum Stress-Associated Apoptosis in HepG2 Cells

Figure 1

Lipid deposition and PNPLA3 expression. The three genotypic HepG2 cells were treated with 0.3 mM PA for 24 h. (a) Visualized oil red O staining using a research-grade inverted microscope at 400x magnification. The scale bar in each image represents 100 µm. (b) Quantification of lipid measured by elution of oil red-O staining. (c) Fold change (PA/BSA) of (b). (d) Quantification of the intracellular TG content. (e) Fold change (PA/BSA) of (d). (f) The protein level of PNPLA3 detected by western blot. (g) The quantitative analysis of gray value based (f) performed using image-pro plus software. (h) Fold change (PA/BSA) of (g). Biological replicates (N = 3) were performed per group. Quantitative data are presented as the mean ± SD (N = 3 independent experiments). ; .

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