PNPLA3 148M/M Is More Susceptible to Palmitic Acid-Induced Endoplasmic Reticulum Stress-Associated Apoptosis in HepG2 Cells

Figure 2

Apoptosis in HepG2 cells and cleaved-caspase-3 expressing. The three genotypic HepG2 cells were treated with 0.3 mM PA for 24 h. (a) The visualized TUNEL staining assay using a research-grade inverted microscope at 400x magnification. The scale bar in each image represents 100 µm. (b) The percentage of TUNEL-positive cells. (c) Fold change (PA/BSA) of (b). (d) The protein level of cleaved-caspase-3 detected by western blot. (e) The quantitative analysis of gray value based (d) performed using image-pro plus software. (f) Fold change (PA/BSA) of (e). Biological replicates (N = 3) were performed per group. Quantitative data are presented as the mean ± SD (N = 3 independent experiments). ; .

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