LncRNA X Inactive Specific Transcript Exerts a Protective Effect on High Glucose-Induced Podocytes by Promoting the Podocyte Autophagy via miR-30d-5p/BECN-1 Axis

Figure 4

XIST targeted miR-30d-5p to regulate apoptosis- and EMT-related proteins in HG-induced podocytes. The experiments were divided into six groups. The first two groups of MPC5 cells were treated with NG or HG. The last four groups of MPC5 cells cotransfected with XIST overexpression plasmid/empty vector and miR-30d-5p mimic/mimic control were treated with HG. (a–d) The expressions of apoptosis-related proteins (Bax, Bcl-2, and cleaved caspase-3) in MPC5 cells were determined by Western blot. GAPDH was used as the internal control. (e–h) The expressions of EMT-related proteins (E-cadherin, Vimentin, and α-SMA) in MPC5 cells were determined by Western blot. GAPDH was used as the internal control. Quantified values were presented as mean ± standard deviation of at least three independent experiments.  ^∧,  ^{∧ ∧ ∧} vs. vector + MC. ^#, ^##, ^### vs. XIST + MC. ^&&, ^&&& vs. vector + M. HG: high glucose. NG: normal glucose. M: mimic. MC: mimic control. EMT: epithelial-mesenchymal transition. GAPDH: glyceraldehyde-3-phosphate dehydrogenase; XIST: X inactive specific transcript.

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