Sildenafil Enhances the Therapeutic Effect of Islet Transplantation for Diabetic Peripheral Neuropathy via mTOR/S6K1 Pathway

Figure 4

Effects of islets on mTOR/S6K1 phosphorylation and expression of BDNF and MBP proteins in RSC96 cells under high glucose conditions. (a) The results of viability assay (CCK-8) of RSC96 cells cultured in high glucose for 24 and 48 hours. (b) Pancreatic islets were cocultured with RSC96 cells. (c, d) The level and semiquantitative analysis of total protein and phosphorylated protein of mTOR/S6K1 in RSC96 cells cocultured with islets under high glucose condition. (e, f) The level and semiquantitative analysis of BDNF and MBP proteins in RSC96 cells cocultured with islets under high glucose condition. (g, h) The analysis of total protein and phosphorylated protein of mTOR/S6K1 in RSC96 cells cocultured with islets following rapamycin pretreatment under high glucose condition. (i, j) The analysis of BDNF and MBP proteins in RSC96 cells cocultured with islets following rapamycin pretreatment under high glucose condition. , , , and .